Question 1

Restriction endonucleases are obtained from various species of bacteria.

Accepted Answer

A) True 
 B)False

Question 2

Which of the following is not true of fluorescent in situ hybridization (FISH) ?

Accepted Answer

A)   Probes can be applied to intact cells.
B)   Probes can locate genes on chromosomes.
C)   It can be used to identify unknown bacteria without culturing.
D)   It uses electrophoresis to separate the DNA.
E)   It can be used to detect RNA in cells.F) A) and B)
G) None of the above

Question 3

DNA fragments can be separated in gel electrophoresis because

Accepted Answer

A)   phosphate groups have a net negative charge.
B)   nitrogenous bases have a net negative charge.
C)   nitrogenous bases have a net positive charge.
D)   phosphate groups have a net positive charge.E) A) and C)
F) None of the above

Question 4

Transgenic animals are referred to as _____ modified organisms.

Accepted Answer

A)   chemically
B)   physically
C)   naturally
D)   geneticallyE) None of the above
F) A) and B)

Question 5

Reverse transcriptase is used to make cDNA from an RNA template.

Accepted Answer

A) True 
 B)False

Question 6

Restriction endonucleases recognize and clip DNA base sequences called ______.

Accepted Answer

A)   codons
B)   palindromes
C)   introns
D)   exons
E)   genesF) A) and D)
G) C) and D)

Question 7

Transformation and transduction are methods used to introduce DNA into host cells.

Accepted Answer

A) True 
 B)False

Question 8

SNPs are derived from ______.

Accepted Answer

A)   genetic engineering
B)   frameshift mutations
C)   gene therapy
D)   point mutationsE) None of the above
F) A) and B)

Question 9

Labeled,known,short stretches of DNA used to detect a specific sequence of nucleotides in a mixture are known as ______.

Accepted Answer

A)   genetic engineering
B)   biotechnology
C)   recombinant DNA
D)   gel electrophoresis
E)   gene probesF) B) and D)
G) C) and D)

Question 10

Microarray analysis is used to

Accepted Answer

A)   analyze an organism's entire genome.
B)   determine which genes are being expressed in an organism.
C)   determine the size of gene fragments after digesting with restriction endonucleases.
D)   analyze an individual's single nucleotide polymorphisms.E) All of the above
F) A) and C)

Question 11

E.coli is the best host for cloning because it possesses the mechanisms for processing and modifying proteins.

Accepted Answer

A) True 
 B)False

Question 12

Which of the following techniques is mismatched with its use in DNA fingerprinting?

Accepted Answer

A)   PCR amplification to get more copies of DNA
B)   Hybridization probes to digest the DNA sample
C)   Electrophoresis to separate DNA fragments
D)   Southern blot for a visual record of DNA fragments
E)   Restriction endonucleases to cut DNAF) A) and E)
G) D) and E)

Question 13

Humans display how much DNA similarity with mice?

Accepted Answer

A)   50%
B)   60%
C)   70%
D)   80%
E)   90%F) A) and C)
G) C) and D)

Question 14

Geneticists can create sequences of DNA from RNA using enzymes called ______.

Accepted Answer

A)   palindromes
B)   reverse transcriptases
C)   restriction endonucleases
D)   ligases
E)   DNA polymerasesF) B) and C)
G) B) and D)

Question 15

Of the following choices,which could be used in the treatment of a patient in order to determine the patient's cancer subtype?

Accepted Answer

A)   Western Blot analysis
B)   PCR
C)   Microarray analysis
D)   Transformation
E)   Oryza sativaF) B) and D)
G) A) and B)

Question 16

Recombinant strains of _____ are released to colonize plant roots to produce an insecticide to destroy invading insects.

Accepted Answer

A)   Pseudomonas syringae
B)   Saccharomyces cerevisiae
C)   Pseudomonas fluorescens
D)   Escherichia coli
E)   Thermus aquaticusF) A) and E)
G) A) and B)

Question 17

Sequences of DNA that are identical when read from the 5'to 3'direction on one strand and the 3'to 5' direction on the other strand are ______.

Accepted Answer

A)   palindromes
B)   reverse transcriptases
C)   restriction endonucleases
D)   ligases
E)   DNA polymerasesF) A) and B)
G) All of the above

Question 18

The first genetically engineered protein approved for human use was ______.

Accepted Answer

A)   human insulin
B)   hemophilia factor VIII
C)   human adrenaline
D)   human testosterone
E)   human growth hormoneF) A) and B)
G) B) and E)

Question 19

Which of the following is a promising treatment for stopping the expression of an unwanted gene?

Accepted Answer

A)   DNA fingerprinting
B)   Antisense therapy
C)   Gene therapy
D)   CloningE) All of the above
F) A) and B)

Question 20

Genetically modified organisms include _____.

Accepted Answer

A)   bacteria
B)   viruses
C)   plants
D)   nonhuman animals
E)   All of the above have been genetically modified.F) D) and E)
G) C) and D)

Exam 10: Genetic Engineering and Recombinant Dna

Of the following choices,which could be used in the treatment of a patient in order to determine the patient's cancer subtype?

Correct AnswerverifiedShow Answer

Humans display how much DNA similarity with mice?

Correct AnswerverifiedShow Answer

Microarray analysis is used to

Correct AnswerverifiedShow Answer

Sequences of DNA that are identical when read from the 5'to 3'direction on one strand and the 3'to 5' direction on the other strand are ______.

Correct AnswerverifiedShow Answer

Recombinant strains of _____ are released to colonize plant roots to produce an insecticide to destroy invading insects.

Correct AnswerverifiedShow Answer

Transformation and transduction are methods used to introduce DNA into host cells.

Correct AnswerverifiedShow Answer

Reverse transcriptase is used to make cDNA from an RNA template.

Correct AnswerverifiedShow Answer

Geneticists can create sequences of DNA from RNA using enzymes called ______.

Correct AnswerverifiedShow Answer

Labeled,known,short stretches of DNA used to detect a specific sequence of nucleotides in a mixture are known as ______.

Correct AnswerverifiedShow Answer

Genetically modified organisms include _____.

Correct AnswerverifiedShow Answer

Transgenic animals are referred to as _____ modified organisms.

Correct AnswerverifiedShow Answer

Restriction endonucleases recognize and clip DNA base sequences called ______.

Correct AnswerverifiedShow Answer

The first genetically engineered protein approved for human use was ______.

Correct AnswerverifiedShow Answer

DNA fragments can be separated in gel electrophoresis because

Correct AnswerverifiedShow Answer

Restriction endonucleases are obtained from various species of bacteria.

Correct AnswerverifiedShow Answer

E.coli is the best host for cloning because it possesses the mechanisms for processing and modifying proteins.

Correct AnswerverifiedShow Answer

SNPs are derived from ______.

Correct AnswerverifiedShow Answer

Which of the following techniques is mismatched with its use in DNA fingerprinting?

Correct AnswerverifiedShow Answer

Which of the following is a promising treatment for stopping the expression of an unwanted gene?

Correct AnswerverifiedShow Answer

Which of the following is not true of fluorescent in situ hybridization (FISH) ?

Correct AnswerverifiedShow Answer

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