Question 1

Thioglycolate medium contains the following: yeast extract, pancreatic digest of casein, glucose, L-cysteine, sodium chloride, sodium thioglycolate, and agar.It is considered a complex medium because

Accepted Answer

A)  depending on their oxygen utilization pattern, organisms will grow differently on it.
B)  it has multiple ingredients for a broad range of nutrients.
C)  both yeast extract and pancreatic digest of casein are not chemically defined.
D)  thioglycolate has a carbon-sulfur bond that many microbes cannot break enzymatically.E) None of the above
F) All of the above

Question 2

Which method often results in colonies developing down throughout the agar along with some colonies on the surface?

Accepted Answer

A)  Spread plate
B)  Pour plate
C)  Replica plate
D)  Streak plateE) None of the above
F) All of the above

Question 3

A nutrient medium that contains at least one ingredient that is not chemically defined is termed ______.

Accepted Answer

A)  enriched
B)  complex
C)  reducing
D)  syntheticE) None of the above
F) A) and B)

Question 4

A selective medium contains one or more substances that inhibit growth of some microbes to facilitate the growth of other desired microbes.

Accepted Answer

A) True 
 B)False

Question 5

Following a properly-performed Gram stain, gram-positive bacteria will appear as pink or red cells.

Accepted Answer

A) True 
 B)False

Question 6

Which of the following is essential for the development of discrete, isolated colonies?

Accepted Answer

A)  Solid medium
B)  Differential medium
C)  Broth medium
D)  Assay medium
E)  Selective mediumF) A) and B)
G) A) and E) A

Question 7

A medium that is gel-like has less agar in it compared to a solid medium.

Accepted Answer

A) True 
 B)False

Question 8

Which isolation technique is most effective for the majority of applications and is most commonly used for colony isolation in the laboratory?

Accepted Answer

A)  Spread plate
B)  Streak plate
C)  Pour plate
D)  Culture plate
E)  Loop dilutionF) A) and C)
G) None of the above

Question 9

A reducing medium contains

Accepted Answer

A)  sugars that can be fermented.
B)  extra oxygen.
C)  hemoglobin, vitamins, or other growth factors.
D)  substances that remove oxygen.
E)  inhibiting agents.F) B) and E)
G) A) and B) D

Question 10

Scattering of peripheral light rays through the glass slide under very high magnification can be prevented by ______.

Accepted Answer

A)  the condenser
B)  the ocular
C)  immersion oil
D)  the cover slipE) A) and B)
F) C) and D)

Question 11

A microbiology student with a visual disability is viewing a sample of Bacillus endospores in a phase contrast microscope that is linked to an iPad.By "stretching" the image on the iPad screen, the student can increase the apparent size of the endospores.How does this manipulation affect resolution and contrast? (No software is being used to alter the image!)

Accepted Answer

A)  Only the magnification can be increased; resolution and contrast are unchanged.
B)  Contrast will improve because the microscope is phase-contrast.
C)  Both magnification and resolution increase; contrast is unchanged.
D)  Resolution and magnification will decrease because the screen is not as good as the human eye.E) C) and D)
F) A) and D)

Question 12

Which type of microscope does not use light in forming the specimen image?

Accepted Answer

A)  Electron
B)  Dark-field
C)  Phase-contrast
D)  Bright-field
E)  FluorescenceF) A) and D)
G) A) and E)

Question 13

Which type of microscope shows cells against a white background?

Accepted Answer

A)  Confocal
B)  Electron
C)  Bright-field
D)  Fluorescence
E)  Phase-contrastF) C) and D)
G) A) and E)

Question 14

Bacteria that require special growth factors and complex nutrients are termed ______.

Accepted Answer

A)  fermentative
B)  hemolytic
C)  fastidious
D)  aerobic
E)  anaerobicF) A) and E)
G) B) and C)

Question 15

A pure culture contains

Accepted Answer

A)  only bacteria.
B)  only one identified species of microorganism.
C)  a variety of species from the same genus.
D)  microbes from a single source.E) A) and D)
F) C) and D)

Question 16

A nutrient medium that has all of its chemical components identified and their precise concentrations known and reproducible is termed ______.

Accepted Answer

A)  reducing
B)  enriched
C)  complex
D)  definedE) None of the above
F) A) and D)

Question 17

The _____ of the microscope holds and allows selection of the objective lenses.

Accepted Answer

A)  ocular
B)  condenser
C)  stage
D)  nosepieceE) All of the above
F) A) and B)

Question 18

The three physical forms of laboratory media are ______.

Accepted Answer

A)  solid, semisolid, and liquid
B)  reducing, transport, and enumeration
C)  a streak plate, a pour plate, and a broth
D)  solid, liquid, and gasE) All of the above
F) B) and C) A

Question 19

Your microscope has a resolving power of 0.15  μ\muμ m.The specimen that you will be observing has bacterial cocci the size of 0.1  μ\muμ m in diameter.Which of the following statements is correct?

Accepted Answer

A)  The cocci will appear much smaller than their actual size because of the low resolution.
B)  The cocci will be fuzzy-looking and blend together.
C)  The cocci will not be visible.
D)  The cocci will be readily visible and distinct. E) C) and D)
F) All of the above

Question 20

The type of microscopy in which you would observe brightly illuminated specimens against a black background is ______.

Accepted Answer

A)  dark-field
B)  confocal
C)  fluorescence
D)  phase-contrast
E)  electronF) A) and C)
G) B) and C)

Exam 3: Tools of the Laboratory: Methods for the Culturing of Microscopic Analysis of Microorganisms

Thioglycolate medium contains the following: yeast extract, pancreatic digest of casein, glucose, L-cysteine, sodium chloride, sodium thioglycolate, and agar.It is considered a complex medium because

Correct Answer
verified

Which method often results in colonies developing down throughout the agar along with some colonies on the surface?

Correct Answer
verified

A nutrient medium that contains at least one ingredient that is not chemically defined is termed ______.

Correct Answer
verified

A selective medium contains one or more substances that inhibit growth of some microbes to facilitate the growth of other desired microbes.

Correct Answer
verified

Following a properly-performed Gram stain, gram-positive bacteria will appear as pink or red cells.

Correct Answer
verified

Which of the following is essential for the development of discrete, isolated colonies?

Correct Answer
verified
A

A medium that is gel-like has less agar in it compared to a solid medium.

Correct Answer
verified

Which isolation technique is most effective for the majority of applications and is most commonly used for colony isolation in the laboratory?

Correct Answer
verified

A reducing medium contains

Correct Answer
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D

Scattering of peripheral light rays through the glass slide under very high magnification can be prevented by ______.

Correct Answer
verified

Correct Answer
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Which type of microscope does not use light in forming the specimen image?

Correct Answer
verified

Which type of microscope shows cells against a white background?

Correct Answer
verified

Bacteria that require special growth factors and complex nutrients are termed ______.

Correct Answer
verified

A pure culture contains

Correct Answer
verified

A nutrient medium that has all of its chemical components identified and their precise concentrations known and reproducible is termed ______.

Correct Answer
verified

The _____ of the microscope holds and allows selection of the objective lenses.

Correct Answer
verified

The three physical forms of laboratory media are ______.

Correct Answer
verified
A

Your microscope has a resolving power of 0.15 $\mu$ m.The specimen that you will be observing has bacterial cocci the size of 0.1 $\mu$ m in diameter.Which of the following statements is correct?

Correct Answer
verified

The type of microscopy in which you would observe brightly illuminated specimens against a black background is ______.

Correct Answer
verified

Exam 3: Tools of the Laboratory: Methods for the Culturing of Microscopic Analysis of Microorganisms

Thioglycolate medium contains the following: yeast extract, pancreatic digest of casein, glucose, L-cysteine, sodium chloride, sodium thioglycolate, and agar.It is considered a complex medium because

Correct AnswerverifiedShow Answer

Which method often results in colonies developing down throughout the agar along with some colonies on the surface?

Correct AnswerverifiedShow Answer

A nutrient medium that contains at least one ingredient that is not chemically defined is termed ______.

Correct AnswerverifiedShow Answer

A selective medium contains one or more substances that inhibit growth of some microbes to facilitate the growth of other desired microbes.

Correct AnswerverifiedShow Answer

Following a properly-performed Gram stain, gram-positive bacteria will appear as pink or red cells.

Correct AnswerverifiedShow Answer

Which of the following is essential for the development of discrete, isolated colonies?

Correct AnswerverifiedA

A medium that is gel-like has less agar in it compared to a solid medium.

Correct AnswerverifiedShow Answer

Which isolation technique is most effective for the majority of applications and is most commonly used for colony isolation in the laboratory?

Correct AnswerverifiedShow Answer

A reducing medium contains

Correct AnswerverifiedD

Scattering of peripheral light rays through the glass slide under very high magnification can be prevented by ______.

Correct AnswerverifiedShow Answer

Correct AnswerverifiedShow Answer

Which type of microscope does not use light in forming the specimen image?

Correct AnswerverifiedShow Answer

Which type of microscope shows cells against a white background?

Correct AnswerverifiedShow Answer

Bacteria that require special growth factors and complex nutrients are termed ______.

Correct AnswerverifiedShow Answer

A pure culture contains

Correct AnswerverifiedShow Answer

A nutrient medium that has all of its chemical components identified and their precise concentrations known and reproducible is termed ______.

Correct AnswerverifiedShow Answer

The _____ of the microscope holds and allows selection of the objective lenses.

Correct AnswerverifiedShow Answer

The three physical forms of laboratory media are ______.

Correct AnswerverifiedA

Your microscope has a resolving power of 0.15 μ\muμ m.The specimen that you will be observing has bacterial cocci the size of 0.1 μ\muμ m in diameter.Which of the following statements is correct?

Correct AnswerverifiedShow Answer

The type of microscopy in which you would observe brightly illuminated specimens against a black background is ______.

Correct AnswerverifiedShow Answer

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A

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D

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A

Your microscope has a resolving power of 0.15 $\mu$ m.The specimen that you will be observing has bacterial cocci the size of 0.1 $\mu$ m in diameter.Which of the following statements is correct?

Correct Answer
verified

Correct Answer
verified