Question 1

Genetically modi?ed organisms include _____.

Accepted Answer

A)   bacteria
B)   viruses
C)   plants
D)   nonhuman animals
E)   All of the above have been genetically modi?ed.F) A) and B)
G) C) and D)

Question 2

Making new genomes is called ______.

Accepted Answer

A)   bioengineering
B)   synthetic biology
C)   genetic engineering
D)   cloning
E)   recombinant DNAF) C) and D)
G) All of the above

Question 3

When DNA is heated, the two strands will separate.

Accepted Answer

A) True 
 B)False

Question 4

Which step in gene cloning involves transformation?

Accepted Answer

A)   Target DNA removed from cells and isolated
B)   Cloning host receives plasmid
C)   Target DNA and plasmid treated with the same restriction endonuclease
D)   Desired protein is produced by cloning host
E)   Gene is ampli?ed by multiplication of cloning hostF) A) and D)
G) B) and E)

Question 5

Vectors often contain a gene conferring drug resistance to their cloning host, in order to detect cells harboring the plasmid.

Accepted Answer

A) True 
 B)False

Question 6

What is the evolutionary advantage of bacteria producing restriction endonucleases?

Accepted Answer

A)   Bacteria use these enzymes to repair their own mistakes made during DNA replication.
B)   These enzymes are a defensive measure of bacteria to defend themselves against invading DNA of bacteriophages.
C)   They make these enzymes for humans to use in manipulating DNA.
D)   Bacteria use these enzymes to attack other bacteria and destroy their DNA.E) A) and B)
F) A) and C)

Question 7

DNA strands can be clipped crosswise at selected positions by using enzymes called ______.

Accepted Answer

A)   palindromes
B)   reverse transcriptases
C)   restriction endonucleases
D)   ligases
E)   DNA polymerasesF) B) and C)
G) A) and E)

Question 8

In the formation of recombinant DNA, what enzyme is needed to seal the sticky ends of genes into plasmids or chromosomes?

Accepted Answer

A)   DNA polymerase I
B)   DNA polymerase II
C)   DNA helicase
D)   DNA ligase
E)   PrimaseF) A) and B)
G) None of the above

Question 9

In choosing the vector used to carry human genes into a host cell, which of the following should be the important consideration?

Accepted Answer

A)   What kind of plasmid it is
B)   Whether the vector comes from a bacterium or a virus
C)   How much donor DNA can be carried on the vector
D)   Whether the vector contains RNA or DNAE) A) and B)
F) None of the above

Question 10

Reverse transcriptase is used to make cDNA from an RNA template.

Accepted Answer

A) True 
 B)False

Question 11

The study of genomes of a particular community is called ______.

Accepted Answer

A)   metabolomics
B)   metagenomics
C)   proteomics
D)   genomology
E)   genomicsF) B) and D)
G) D) and E)

Question 12

Sequences of DNA that are identical when read from the 5' to 3' direction on one strand and the 3' to 5' direction on the other strand are ______.

Accepted Answer

A)   palindromes
B)   reverse transcriptases
C)   restriction endonucleases
D)   ligases
E)   DNA polymerasesF) B) and D)
G) All of the above

Question 13

If you start with 3 double-stranded DNA fragments, after 4 cycles of PCR you will have ______ fragments.

Accepted Answer

A)   12
B)   24
C)   27
D)   48
E)   81F) All of the above
G) C) and D)

Question 14

Often natural disasters leave little intact DNA with which to identify the victim's remains using traditional DNA ?ngerprinting techniques. The discovery of ______ made genetic analysis possible with minute amounts of genetic material.

Accepted Answer

A)   frameshift mutations
B)   SNPs
C)   microarrays
D)   miRNAsE) A) and B)
F) A) and C)

Question 15

Geneticists can create sequences of DNA from RNA using enzymes called ______.

Accepted Answer

A)   palindromes
B)   reverse transcriptases
C)   restriction endonucleases
D)   ligases
E)   DNA polymerasesF) C) and D)
G) A) and B)

Question 16

Restriction endonucleases are obtained from various species of bacteria.

Accepted Answer

A) True 
 B)False

Question 17

Transgenic organisms are ______.

Accepted Answer

A)   created in nature
B)   only microorganisms
C)   copyrighted
D)   patentedE) All of the above
F) C) and D)

Question 18

Microarray analysis is used to ______.

Accepted Answer

A)   analyze an individual's single nucleotide polymorphisms
B)   analyze an organism's entire genome
C)   determine the size of gene fragments after digesting with restriction endonucleases
D)   determine which genes are being expressed in an organismE) A) and B)
F) C) and D)

Question 19

Viruses are often used as cloning hosts in recombinant DNA methods.

Accepted Answer

A) True 
 B)False

Question 20

You want to identify Mycobacterium tuberculosis in a patient sputum sample. Which procedure would be most useful in this case?

Accepted Answer

A)   Whole genome sequencing
B)   Microarray analysis
C)   Polymerase chain reaction
D)   DNA probe analysisE) A) and B)
F) B) and C)

Exam 10: Genetic Analysis and Genetic Engineering

What is the evolutionary advantage of bacteria producing restriction endonucleases?

Correct AnswerverifiedShow Answer

Viruses are often used as cloning hosts in recombinant DNA methods.

Correct AnswerverifiedShow Answer

Making new genomes is called ______.

Correct AnswerverifiedShow Answer

The study of genomes of a particular community is called ______.

Correct AnswerverifiedShow Answer

You want to identify Mycobacterium tuberculosis in a patient sputum sample. Which procedure would be most useful in this case?

Correct AnswerverifiedShow Answer

Geneticists can create sequences of DNA from RNA using enzymes called ______.

Correct AnswerverifiedShow Answer

Transgenic organisms are ______.

Correct AnswerverifiedShow Answer

Reverse transcriptase is used to make cDNA from an RNA template.

Correct AnswerverifiedShow Answer

Often natural disasters leave little intact DNA with which to identify the victim's remains using traditional DNA ?ngerprinting techniques. The discovery of ______ made genetic analysis possible with minute amounts of genetic material.

Correct AnswerverifiedShow Answer

Sequences of DNA that are identical when read from the 5' to 3' direction on one strand and the 3' to 5' direction on the other strand are ______.

Correct AnswerverifiedShow Answer

Restriction endonucleases are obtained from various species of bacteria.

Correct AnswerverifiedShow Answer

Which step in gene cloning involves transformation?

Correct AnswerverifiedShow Answer

In the formation of recombinant DNA, what enzyme is needed to seal the sticky ends of genes into plasmids or chromosomes?

Correct AnswerverifiedShow Answer

Microarray analysis is used to ______.

Correct AnswerverifiedShow Answer

In choosing the vector used to carry human genes into a host cell, which of the following should be the important consideration?

Correct AnswerverifiedShow Answer

If you start with 3 double-stranded DNA fragments, after 4 cycles of PCR you will have ______ fragments.

Correct AnswerverifiedShow Answer

When DNA is heated, the two strands will separate.

Correct AnswerverifiedShow Answer

Vectors often contain a gene conferring drug resistance to their cloning host, in order to detect cells harboring the plasmid.

Correct AnswerverifiedShow Answer

DNA strands can be clipped crosswise at selected positions by using enzymes called ______.

Correct AnswerverifiedShow Answer

Genetically modi?ed organisms include _____.

Correct AnswerverifiedShow Answer

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